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An Advanced Tape-Stripping Approach for High-Efficiency Sampling on Non-Absorbent Surfaces

Author

Listed:
  • Pengcheng Zhao

    (Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR 999077, China)

  • Pak-To Chan

    (Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR 999077, China)

  • Nan Zhang

    (Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR 999077, China
    Beijing Key Laboratory of Green Built Environment and Energy Efficient Technology, Beijing University of Technology, Beijing 100124, China)

  • Yuguo Li

    (Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR 999077, China
    School of Public Health, The University of Hong Kong, Hong Kong SAR 999077, China)

Abstract

Surface sampling is a frequent task in laboratory work and field studies. Simple methods usually have low efficiency in collecting target substances from surfaces. This study developed an advanced tape-stripping approach for efficient sampling on non-absorbent surfaces. A film-forming solution, prepared using polyvinyl alcohol, is applied to the target surface, where it covers and engulfs the surface deposits and then solidifies into an elastic membrane as it is exposed to air. The deposits are collected by stripping off the membrane and re-dissolving it in water. This new approach exhibited an efficiency of 100% in collecting uniform-size microspheres from glass surfaces and extremely high efficiencies (>96.6%) in detecting selected target DNA materials from glass and stainless steel surfaces. In comparison, the common swab-rinse method exhibited an efficiency of 72.6% under similar measuring conditions. The viability of S. aureus during sampling using the new approach decreased as the ethanol concentration in the applied solution increased. Using a solution with a mass ratio of ethanol of 17.6% balanced the effects of multiplication and degradation of the S. aureus on glass surfaces during sampling. Overall, the proposed approach exhibits high efficiency in collecting living and abiotic matter from non-absorbent surfaces, complementing existing sampling methods.

Suggested Citation

  • Pengcheng Zhao & Pak-To Chan & Nan Zhang & Yuguo Li, 2022. "An Advanced Tape-Stripping Approach for High-Efficiency Sampling on Non-Absorbent Surfaces," IJERPH, MDPI, vol. 19(19), pages 1-16, October.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:19:p:12571-:d:931632
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    References listed on IDEAS

    as
    1. Rached Ismaïl & Florence Aviat & Valérie Michel & Isabelle Le Bayon & Perrine Gay-Perret & Magdalena Kutnik & Michel Fédérighi, 2013. "Methods for Recovering Microorganisms from Solid Surfaces Used in the Food Industry: A Review of the Literature," IJERPH, MDPI, vol. 10(11), pages 1-15, November.
    2. Natalie G. Exum & Margaret N. Kosek & Meghan F. Davis & Kellogg J. Schwab, 2017. "Surface Sampling Collection and Culture Methods for Escherichia coli in Household Environments with High Fecal Contamination," IJERPH, MDPI, vol. 14(8), pages 1-9, August.
    Full references (including those not matched with items on IDEAS)

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