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γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of Bacillus subtilis A-5 and the Complete Genome Analysis

Author

Listed:
  • Naling Bai

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

  • Yu He

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

  • Hanlin Zhang

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

  • Xianqing Zheng

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

  • Rong Zeng

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

  • Yi Li

    (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

  • Shuangxi Li

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
    Agricultural Environment and Farmland Conservation Experiment Station, Ministry Agriculture and Rural Affairs, Shanghai 201403, China
    Key Laboratory of Low-Carbon Green Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China)

  • Weiguang Lv

    (Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
    Agricultural Environment and Farmland Conservation Experiment Station, Ministry Agriculture and Rural Affairs, Shanghai 201403, China
    Key Laboratory of Low-Carbon Green Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China
    Shanghai Key Laboratory of Horticultural Technology, Shanghai 201403, China)

Abstract

Bacillus subtilis A-5 has the capabilities of high-molecular-weight γ-PGA production, antagonism to plant pathogenic fungi, and salt/alkaline tolerance. This multifunctional bacterium has great potential for enhancing soil fertility and plant security in agricultural ecosystem. The genome size of B. subtilis A-5 was 4,190,775 bp, containing 1 Chr and 2 plasmids (pA and pB) with 43.37% guanine-cytosine content and 4605 coding sequences. The γ-PGA synthase gene cluster was predicted to consist of pgsBCA and factor ( pgsE ). The γ-PGA-degrading enzymes were mainly pgdS, GGT, and cwlO. Nine gene clusters producing secondary metabolite substances, namely, four unknown function gene clusters and five antibiotic synthesis gene clusters (surfactin, fengycin, bacillibactin, subtilosin_A, and bacilysin), were predicted in the genome of B. subtilis A-5 using antiSMASH. In addition, B. subtilis A-5 contained genes related to carbohydrate and protein decomposition, proline synthesis, pyruvate kinase, and stress-resistant proteins. This affords significant insights into the survival and application of B. subtilis A-5 in adverse agricultural environmental conditions.

Suggested Citation

  • Naling Bai & Yu He & Hanlin Zhang & Xianqing Zheng & Rong Zeng & Yi Li & Shuangxi Li & Weiguang Lv, 2022. "γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of Bacillus subtilis A-5 and the Complete Genome Analysis," IJERPH, MDPI, vol. 19(13), pages 1-17, June.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:13:p:7630-:d:844996
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    References listed on IDEAS

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    1. Shehzad Mehmood & Amir Abdullah Khan & Fuchen Shi & Muhammad Tahir & Tariq Sultan & Muhammad Farooq Hussain Munis & Prashant Kaushik & Mohammed Nasser Alyemeni & Hassan Javed Chaudhary, 2021. "Alleviation of Salt Stress in Wheat Seedlings via Multifunctional Bacillus aryabhattai PM34: An In-Vitro Study," Sustainability, MDPI, vol. 13(14), pages 1-13, July.
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