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The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori

Author

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  • Ji Yeong Yang

    (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea
    Ji Yeong Yang and Pumsoo Kim contributed equally to this work.)

  • Pumsoo Kim

    (Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St. Mary’s Hospital, Incheon 22711, Korea
    Ji Yeong Yang and Pumsoo Kim contributed equally to this work.)

  • Seok-Hoo Jeong

    (Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St. Mary’s Hospital, Incheon 22711, Korea)

  • Seong Woong Lee

    (Samil Pharmaceutical Co. Ltd., Seoul 06666, Korea)

  • Yu Sik Myung

    (Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St. Mary’s Hospital, Incheon 22711, Korea)

  • Myong Ki Baeg

    (Division of Gastroenterology, Department of Internal Medicine, Catholic Kwandong University International St. Mary’s Hospital, Incheon 22711, Korea)

  • Jong-Bae Kim

    (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea)

Abstract

Helicobacter pylori ( H. pylori ) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after H. pylori infection in human gastric adenocarcinoma cells (AGS cells). H. pylori reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of H. pylori was evaluated using the broth dilution method. Inhibition of H. pylori adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of H. pylori , however, after six and 12 hours of infection on AGS cells, H. pylori adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased H. pylori virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited H. pylori adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat H. pylori infections.

Suggested Citation

  • Ji Yeong Yang & Pumsoo Kim & Seok-Hoo Jeong & Seong Woong Lee & Yu Sik Myung & Myong Ki Baeg & Jong-Bae Kim, 2020. "The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori," IJERPH, MDPI, vol. 17(8), pages 1-9, April.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:8:p:2918-:d:349321
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    References listed on IDEAS

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    1. Iraj Saadat & Hideaki Higashi & Chikashi Obuse & Mayumi Umeda & Naoko Murata-Kamiya & Yasuhiro Saito & Huaisheng Lu & Naomi Ohnishi & Takeshi Azuma & Atsushi Suzuki & Shigeo Ohno & Masanori Hatakeyama, 2007. "Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity," Nature, Nature, vol. 447(7142), pages 330-333, May.
    2. Emad M. El-Omar & Mary Carrington & Wong-Ho Chow & Kenneth E. L. McColl & Jay H. Bream & Howard A. Young & Jesus Herrera & Jolanta Lissowska & Chiu-Chin Yuan & Nathaniel Rothman & George Lanyon & Maur, 2000. "Interleukin-1 polymorphisms associated with increased risk of gastric cancer," Nature, Nature, vol. 404(6776), pages 398-402, March.
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