IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v15y2022i5p1920-d765310.html
   My bibliography  Save this article

Metagenomic Analysis of the Long-Term Synergistic Effects of Antibiotics on the Anaerobic Digestion of Cattle Manure

Author

Listed:
  • Izabela Wolak

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Małgorzata Czatzkowska

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Monika Harnisz

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

  • Jan Paweł Jastrzębski

    (Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-957 Olsztyn, Poland)

  • Łukasz Paukszto

    (Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-721 Olsztyn, Poland)

  • Paulina Rusanowska

    (Department of Environmental Engineering, University of Warmia and Mazury in Olsztyn, Warszawska 117, 10-950 Olsztyn, Poland)

  • Ewa Felis

    (Department of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2, 44-100 Gliwice, Poland)

  • Ewa Korzeniewska

    (Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland)

Abstract

The conversion of cattle manure into biogas in anaerobic digestion (AD) processes has been gaining attention in recent years. However, antibiotic consumption continues to increase worldwide, which is why antimicrobial concentrations can be expected to rise in cattle manure and in digestate. This study examined the long-term synergistic effects of antimicrobials on the anaerobic digestion of cattle manure. The prevalence of antibiotic resistance genes (ARGs) and changes in microbial biodiversity under exposure to the tested drugs was investigated using a metagenomic approach. Methane production was analyzed in lab-scale anaerobic bioreactors. Bacteroidetes , Firmicutes, and Actinobacteria were the most abundant bacteria in the samples. The domain Archaea was represented mainly by methanogenic genera Methanothrix and Methanosarcina and the order Methanomassiliicoccales . Exposure to antibiotics inhibited the growth and development of methanogenic microorganisms in the substrate. Antibiotics also influenced the abundance and prevalence of ARGs in samples. Seventeen types of ARGs were identified and classified. Genes encoding resistance to tetracyclines, macrolide–lincosamide–streptogramin antibiotics, and aminoglycosides, as well as multi-drug resistance genes, were most abundant. Antibiotics affected homoacetogenic bacteria and methanogens, and decreased the production of CH 4 . However, the antibiotic-induced decrease in CH 4 production was minimized in the presence of highly drug-resistant microorganisms in AD bioreactors.

Suggested Citation

  • Izabela Wolak & Małgorzata Czatzkowska & Monika Harnisz & Jan Paweł Jastrzębski & Łukasz Paukszto & Paulina Rusanowska & Ewa Felis & Ewa Korzeniewska, 2022. "Metagenomic Analysis of the Long-Term Synergistic Effects of Antibiotics on the Anaerobic Digestion of Cattle Manure," Energies, MDPI, vol. 15(5), pages 1-19, March.
  • Handle: RePEc:gam:jeners:v:15:y:2022:i:5:p:1920-:d:765310
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/15/5/1920/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1996-1073/15/5/1920/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Izabela Koniuszewska & Monika Harnisz & Ewa Korzeniewska & Małgorzata Czatzkowska & Jan Paweł Jastrzębski & Łukasz Paukszto & Sylwia Bajkacz & Ewa Felis & Paulina Rusanowska, 2021. "The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure," Energies, MDPI, vol. 14(4), pages 1-19, February.
    2. Chris S. Smillie & Mark B. Smith & Jonathan Friedman & Otto X. Cordero & Lawrence A. David & Eric J. Alm, 2011. "Ecology drives a global network of gene exchange connecting the human microbiome," Nature, Nature, vol. 480(7376), pages 241-244, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Izabela Wolak & Sylwia Bajkacz & Monika Harnisz & Klaudia Stando & Magdalena Męcik & Ewa Korzeniewska, 2023. "Digestate from Agricultural Biogas Plants as a Reservoir of Antimicrobials and Antibiotic Resistance Genes—Implications for the Environment," IJERPH, MDPI, vol. 20(3), pages 1-19, February.
    2. Charles K Fisher & Thierry Mora & Aleksandra M Walczak, 2017. "Variable habitat conditions drive species covariation in the human microbiota," PLOS Computational Biology, Public Library of Science, vol. 13(4), pages 1-18, April.
    3. Patrick Munk & Christian Brinch & Frederik Duus Møller & Thomas N. Petersen & Rene S. Hendriksen & Anne Mette Seyfarth & Jette S. Kjeldgaard & Christina Aaby Svendsen & Bram Bunnik & Fanny Berglund & , 2022. "Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Nils Giordano & Marinna Gaudin & Camille Trottier & Erwan Delage & Charlotte Nef & Chris Bowler & Samuel Chaffron, 2024. "Genome-scale community modelling reveals conserved metabolic cross-feedings in epipelagic bacterioplankton communities," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Rohan Maddamsetti & Yi Yao & Teng Wang & Junheng Gao & Vincent T. Huang & Grayson S. Hamrick & Hye-In Son & Lingchong You, 2024. "Duplicated antibiotic resistance genes reveal ongoing selection and horizontal gene transfer in bacteria," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    6. Samuel C. Forster & Junyan Liu & Nitin Kumar & Emily L. Gulliver & Jodee A. Gould & Alejandra Escobar-Zepeda & Tapoka Mkandawire & Lindsay J. Pike & Yan Shao & Mark D. Stares & Hilary P. Browne & B. A, 2022. "Strain-level characterization of broad host range mobile genetic elements transferring antibiotic resistance from the human microbiome," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    7. Kihyun Lee & Sebastien Raguideau & Kimmo Sirén & Francesco Asnicar & Fabio Cumbo & Falk Hildebrand & Nicola Segata & Chang-Jun Cha & Christopher Quince, 2023. "Population-level impacts of antibiotic usage on the human gut microbiome," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    8. Paulo Martins Da Costa & Luís Loureiro & Augusto J. F. Matos, 2013. "Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment," IJERPH, MDPI, vol. 10(1), pages 1-17, January.
    9. Eugen Pfeifer & Eduardo P. C. Rocha, 2024. "Phage-plasmids promote recombination and emergence of phages and plasmids," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    10. Peter J. Diebold & Matthew W. Rhee & Qiaojuan Shi & Nguyen Vinh Trung & Fayaz Umrani & Sheraz Ahmed & Vandana Kulkarni & Prasad Deshpande & Mallika Alexander & Ngo Hoa & Nicholas A. Christakis & Najee, 2023. "Clinically relevant antibiotic resistance genes are linked to a limited set of taxa within gut microbiome worldwide," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    11. Qihang Shu & Hongkuan Cheng & Xiaxia Chen & Jie Wang & Zunqing Du & Jun Hong & Zheng Zheng & Xingzhang Luo, 2022. "Effects of Increasing Concentrations of Enrofloxacin on Co-Digestion of Pig Manure and Corn Straw," Sustainability, MDPI, vol. 14(10), pages 1-14, May.
    12. Małgorzata Czatzkowska & Izabela Wolak & Monika Harnisz & Ewa Korzeniewska, 2022. "Impact of Anthropogenic Activities on the Dissemination of ARGs in the Environment—A Review," IJERPH, MDPI, vol. 19(19), pages 1-29, October.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:15:y:2022:i:5:p:1920-:d:765310. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.