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DNA Methylome and Transcriptome Maps of Primary Colorectal Cancer and Matched Liver Metastasis

Author

Listed:
  • Priyadarshana Ajithkumar

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Gregory Gimenez

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Peter A. Stockwell

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Suzan Almomani

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Sarah A. Bowden

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Anna L. Leichter

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Antonio Ahn

    (Peter MacCallum Cancer Centre, Melbourne, VIC 3052, Australia
    The Sir Peter MacCallum Department of Oncology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, VIC 3052, Australia)

  • Sharon Pattison

    (Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Sebastian Schmeier

    (Evotec SE, 22419 Hamburg, Germany)

  • Frank A. Frizelle

    (Department of Surgery, University of Otago, Christchurch 8011, New Zealand)

  • Michael R. Eccles

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Rachel V. Purcell

    (Department of Surgery, University of Otago, Christchurch 8011, New Zealand)

  • Euan J. Rodger

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand)

  • Aniruddha Chatterjee

    (Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
    UPES University, Dehradun 248007, India)

Abstract

Sequencing-based genome-wide DNA methylation, gene expression studies and associated data on paired colorectal cancer (CRC) primary and liver metastasis are very limited. We have profiled the DNA methylome and transcriptome of matched primary CRC and liver metastasis samples from the same patients. Genome-scale methylation and expression levels were examined using Reduced Representation Bisulfite Sequencing (RRBS) and RNA-Seq, respectively. To investigate DNA methylation and expression patterns, we generated a total of 1.01 × 10 9 RRBS reads and 4.38 × 10 8 RNA-Seq reads from the matched cancer tissues. Here, we describe in detail the sample features, experimental design, methods and bioinformatic pipeline for these epigenetic data. We demonstrate the quality of both the samples and sequence data obtained from the paired samples. The sequencing data obtained from this study will serve as a valuable resource for studying underlying mechanisms of distant metastasis and the utility of epigenetic profiles in cancer metastasis.

Suggested Citation

  • Priyadarshana Ajithkumar & Gregory Gimenez & Peter A. Stockwell & Suzan Almomani & Sarah A. Bowden & Anna L. Leichter & Antonio Ahn & Sharon Pattison & Sebastian Schmeier & Frank A. Frizelle & Michael, 2023. "DNA Methylome and Transcriptome Maps of Primary Colorectal Cancer and Matched Liver Metastasis," Data, MDPI, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:gam:jdataj:v:9:y:2023:i:1:p:8-:d:1310308
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