IDEAS home Printed from https://ideas.repec.org/a/gam/jdataj/v8y2023i12p177-d1287468.html
   My bibliography  Save this article

Dataset: Impact of β-Galactosylceramidase Overexpression on the Protein Profile of Braf(V600E) Mutated Melanoma Cells

Author

Listed:
  • Davide Capoferri

    (Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy)

  • Paola Chiodelli

    (Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
    Present address: Dipartimento di Scienze della Vita e Sanità Pubblica, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.)

  • Stefano Calza

    (Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy)

  • Marcello Manfredi

    (Department of Translational Medicine, University of Piemonte Orientale, 13100 Novara, Italy
    Center for Allergic and Autoimmune Diseases, University of Piemonte Orientale, 13100 Novara, Italy)

  • Marco Presta

    (Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
    Consorzio Interuniversitario Biotecnologie (CIB), Unit of Brescia, 25123 Brescia, Italy)

Abstract

β-Galactosylceramidase (GALC) is a lysosomal enzyme involved in sphingolipid metabolism by removing β-galactosyl moieties from β-galactosyl ceramide and β-galactosyl sphingosine. Previous observations have shown that GALC exerts a pro-oncogenic activity in human melanoma. Here, the impact of GALC overexpression on the proteomic landscape of BRAF -mutated A2058 and A375 human melanoma cell lines was investigated by liquid chromatography–tandem mass spectrometry analysis of the cell extracts. The results indicate that GALC overexpression causes the upregulation/downregulation of 172/99 proteins in GALC -transduced cells when compared to control cells. Gene ontology categorization of up/down-regulated proteins indicates that GALC may modulate the protein landscape in BRAF -mutated melanoma cells by affecting various biological processes, including RNA metabolism, cell organelle fate, and intracellular redox status. Overall, these data provide further insights into the pro-oncogenic functions of the sphingolipid metabolizing enzyme GALC in human melanoma.

Suggested Citation

  • Davide Capoferri & Paola Chiodelli & Stefano Calza & Marcello Manfredi & Marco Presta, 2023. "Dataset: Impact of β-Galactosylceramidase Overexpression on the Protein Profile of Braf(V600E) Mutated Melanoma Cells," Data, MDPI, vol. 8(12), pages 1-7, November.
    • Handle: RePEc:gam:jdataj:v:8:y:2023:i:12:p:177-:d:1287468
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2306-5729/8/12/177/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2306-5729/8/12/177/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jdataj:v:8:y:2023:i:12:p:177-:d:1287468. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.