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Genome Survey of Stipa breviflora Griseb. Using Next-Generation Sequencing

Author

Listed:
  • Xiangjun Yun

    (Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
    These authors contributed equally to this work.)

  • Jinrui Wu

    (Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
    These authors contributed equally to this work.)

  • Bo Xu

    (Inner Mongolia Forestry Monitoring and Planning Institute, Hohhot 010020, China)

  • Shijie Lv

    (College of Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Le Zhang

    (Inner Mongolia Forestry Monitoring and Planning Institute, Hohhot 010020, China)

  • Wenguang Zhang

    (College of Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Shixian Sun

    (Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China)

  • Guixiang Liu

    (Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China)

  • Yazhou Zu

    (Nei Mongol BioNew Technology, Hohhot 010018, China)

  • Bin Liu

    (Nei Mongol BioNew Technology, Hohhot 010018, China)

Abstract

Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora , a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. To the best of our knowledge, the present study is the first to sequence, assemble, and annotate the S. breviflora genome. In total, 2,781,544 contigs were assembled, and 2,600,873 scaffolds were obtained, resulting in a total length of 649,849,683 bp. The number of scaffolds greater than 1 kb was 70,770. We annotated the assembled genome (>121 kb), conducted a selective sweep analysis, and ultimately succeeded in assembling the Matk gene of S. breviflora . More importantly, our research identified 26 scaffolds that may be responsible for the drought tolerance of S. breviflora Griseb. In summary, the data obtained regarding S. breviflora will be of great significance for future research.

Suggested Citation

  • Xiangjun Yun & Jinrui Wu & Bo Xu & Shijie Lv & Le Zhang & Wenguang Zhang & Shixian Sun & Guixiang Liu & Yazhou Zu & Bin Liu, 2023. "Genome Survey of Stipa breviflora Griseb. Using Next-Generation Sequencing," Agriculture, MDPI, vol. 13(12), pages 1-16, December.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:12:p:2243-:d:1294220
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    References listed on IDEAS

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    1. Carl-Johan Rubin & Michael C. Zody & Jonas Eriksson & Jennifer R. S. Meadows & Ellen Sherwood & Matthew T. Webster & Lin Jiang & Max Ingman & Ted Sharpe & Sojeong Ka & Finn Hallböök & Francois Besnier, 2010. "Whole-genome resequencing reveals loci under selection during chicken domestication," Nature, Nature, vol. 464(7288), pages 587-591, March.
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