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Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis

Author

Listed:
  • M Zouharova

    (Infectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech Republic)

  • K Nedbalcova

    (Infectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech Republic)

  • P Slama

    (Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic)

  • J Bzdil

    (Microbiologist and Practical Veterinarian, Ptácy s.r.o., Valašská Bystřice, Czech Republic)

  • M Masarikova

    (Department of Infectious Diseases and Microbiology, University of Veterinary Sciences, Brno, Czech Republic)

  • J Matiasovic

    (Infectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech Republic)

Abstract

Streptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in S. uberis isolated from subclinical or clinical mastitis and its closely related species Streptococcus parauberis in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and most isolates carried eight out of ten virulence genes. All S. uberis isolates were positive for the oppF, gapC and sua genes. Among the most prevalent virulence-associated genes skc (98%) and pauA (97%) were also found. The hasA and hasB genes were always present together in 94% of the isolates. The genes cfu and lbp were detected in 6% and 2%, respectively. In the S. uberis isolates, 14 different virulence gene profiles were observed. The most frequent profile was hasA+ hasB+ sua+ skc+ pauA+ gapC+ oppF with variable hasC, observed in 86% of the tested isolates, occurring in 127 out of 135 farms. S. parauberis was identified very sporadically and, although it is closely related to S. uberis, only a rare occurrence of the examined virulence-associated genes was found.

Suggested Citation

  • M Zouharova & K Nedbalcova & P Slama & J Bzdil & M Masarikova & J Matiasovic, 2022. "Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 67(3), pages 123-130.
    • Handle: RePEc:caa:jnlvet:v:67:y:2022:i:3:id:95-2021-vetmed
    DOI: 10.17221/95/2021-VETMED
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