IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v62y2017i10id14-2017-vetmed.html
   My bibliography  Save this article

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Author

Listed:
  • A. Rehman

    (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • R. Akhtar

    (Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • H. Akbar

    (Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • F. Riaz

    (Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China)

  • I. Rashid

    (Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • W. Shehzad

    (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • S. Islam

    (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • A.A. Bajwa

    (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • M. Waqas

    (Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan)

Abstract

The molecular prevalence of Ancylostoma caninum was determined in stray and pet dogs in Lahore, Pakistan from July 2014 to August 2015. A total of 500 dog faecal samples were first evaluated using a sedimentation technique and further through a PCR assay targeting the ITS-2 region of the A. caninum genome. Overall, 130 (26%) samples were positive for hookworm infestation by microscopic examination. Only microscopically positive samples were processed for PCR and 89 (17.6%) were positive for A. caninum. Sequence analysis of amplicons showed 100% homology with A. caninum and the genotypes clustered in one clade with Brazilian A. caninum hookworms. There was a significantly (P < 0.05) increased prevalence in male dogs younger than six months old. Labrador retrievers had higher A. caninum incidence compared to German shepherds and other breeds. To the best of our knowledge, this is the first report of the molecular prevalence of A. caninum in dogs in Pakistan. The novel results of the present study allow us to conclude that A. caninum is more prevalent in pet dogs, especially puppies, and this can be a potential threat for humans that come into contact with such animals. Therefore, the routine monitoring of pets, especially the more susceptible breeds, is essential for disease control.

Suggested Citation

  • A. Rehman & R. Akhtar & H. Akbar & F. Riaz & I. Rashid & W. Shehzad & S. Islam & A.A. Bajwa & M. Waqas, 2017. "First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 62(10), pages 559-564.
    • Handle: RePEc:caa:jnlvet:v:62:y:2017:i:10:id:14-2017-vetmed
    DOI: 10.17221/14/2017-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/14/2017-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/14/2017-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/14/2017-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:62:y:2017:i:10:id:14-2017-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.