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Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples

Author

Listed:
  • E. Atil

    (Pendik Veterinary Control and Research Institute, Istanbul, Turkey)

  • H.B. Ertas

    (Faculty of Veterinary Medicine, Firat University, Elazig, Turkey)

  • G. Ozbey

    (Vocational School of Health Services, Firat University, Elazig, Turkey)

Abstract

A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Listeria grayi (15.2%) and to Listeria welshimeri (11%). No Listeria ivanovii were isolated from any of the samples. Listeria spp. were not isolated from cheese and bulk tank swabs. With regard to seasonal variations most Listeria spp. were isolated in the spring and winter seasons. The eight L. monocytogenes isolates were characterized by PCR-RFLP with AluI and Tsp509I. RFLP typing of the isolates revealed two different profiles with both restriction enzymes. Four and six different profiles were produced in the examination of L. monocytogenes isolates with RAPD analysis with HLWL74 and HLWL85 primers, respectively. This is the first report on the genotyping of L. monocytogenes isolates from various sources in Turkey. This study has highlighted the need for improved control and epidemiologic strategies to prevent the transmission of Listeria spp. to animals and humans.

Suggested Citation

  • E. Atil & H.B. Ertas & G. Ozbey, 2011. "Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 56(8), pages 386-394.
    • Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1551-vetmed
    DOI: 10.17221/1551-VETMED
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    Cited by:

    1. Imran Khan & Jangrez Khan & Sumaira Miskeen & Charles Nkufi Tango & Youn-Seo Park & Deog-Hwan Oh, 2016. "Prevalence and control of Listeria monocytogenes in the food industry - a review," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 34(6), pages 469-487.

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