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Appearance of iron-labeled blood mononuclear cells in electron microscopy

Author

Listed:
  • D. Horky

    (Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic)

  • I. Lauschova

    (Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic)

  • M. Klabusay

    (Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic)

  • M. Doubek

    (Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic)

  • P. Sheer

    (Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic)

  • S. Palsa

    (Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic)

  • J. Doubek

    (Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic)

Abstract

Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.

Suggested Citation

  • D. Horky & I. Lauschova & M. Klabusay & M. Doubek & P. Sheer & S. Palsa & J. Doubek, 2006. "Appearance of iron-labeled blood mononuclear cells in electron microscopy," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(3), pages 89-92.
    • Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5525-vetmed
    DOI: 10.17221/5525-VETMED
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