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An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli

Author

Listed:
  • D.Y. Zhang

    (College of Life Science,
    Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,)

  • J.Y. Zhou

    (Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,)

  • J. Fang

    (Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,)

  • J.Q. HU

    (Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,)

  • J.X. WU

    (Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
    Institute of Biotechnology, Zhejiang University, Hangzhou, China)

  • A.X. MU

    (General Station of Veterinary Medicine, Hangzhou Bureau of Agriculture, Hangzhou, China)

Abstract

The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure.

Suggested Citation

  • D.Y. Zhang & J.Y. Zhou & J. Fang & J.Q. HU & J.X. WU & A.X. MU, 2005. "An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(8), pages 336-344.
    • Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5632-vetmed
    DOI: 10.17221/5632-VETMED
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    Cited by:

    1. D.Y. Zhang & J.Y. Zhou & W.Q. Chen & J.G. Chen, 2009. "Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(4), pages 169-174.

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