IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpps/v58y2022i4id46-2022-pps.html
   My bibliography  Save this article

Biological impact, oxidative stress and adipokinetic hormone activities of Agrotis ipsilon in response to bioinsecticides

Author

Listed:
  • Moataz A.M. Moustafa

    (Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt)

  • Wael H. Elmenofy

    (Agricultural Genetic Engineering Research Institute, ARC, Giza, Egypt)

  • Engy A. Osman

    (Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt)

  • Nourhan A. El-Said

    (Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt)

  • Mona Awad

    (Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt)

Abstract

The use of biological control products enhances the reduction of harmful pressures on the environment caused by the use of conventional pesticides. Therefore, this study aims to evaluate the toxicity of eight bioinsecticides on the black cutworm Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) to understand the relationships between insecticide exposure and insect response at the individual and population levels. The bioassay results showed that emamectin benzoate had high toxicity against A. ipsilon second instar larvae with an LC50 of 0.007 (mg/L). Sublethal concentration (LC30) of emamectin benzoate, spinosyn group and Bacillus thuringiensis formulation (Dipel 2X) had significantly prolonged pupal period. In addition, emamectin benzoate significantly decreased the number of eggs laid per female (135.3 ± 6.919) compared with the control treatment. Thus, the formulation of chlorfenapyr showed a 1.26-fold reduction in hatching rate. Meanwhile, the activities of oxidative stress enzymes (catalase, superoxide dismutase, lipid peroxidase, and glutathione reductase) were significantly affected due to exposure to sublethal concentration. Changes in adipokinetic hormone (AKH) transcriptional regulation were detected via SQ-PCR via using cDNA synthesized from mRNA isolated from treated A. ipsilon larvae. The results showed a higher transcription rate of AKH in spinotram-treated larvae with a 1.42-fold increase over untreated larvae. Our results provide useful information for integrated pest management programs for A. ipsilon by using bioinsecticides.

Suggested Citation

  • Moataz A.M. Moustafa & Wael H. Elmenofy & Engy A. Osman & Nourhan A. El-Said & Mona Awad, 2022. "Biological impact, oxidative stress and adipokinetic hormone activities of Agrotis ipsilon in response to bioinsecticides," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 58(4), pages 326-337.
  • Handle: RePEc:caa:jnlpps:v:58:y:2022:i:4:id:46-2022-pps
    DOI: 10.17221/46/2022-PPS
    as

    Download full text from publisher

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/46/2022-PPS.html
    Download Restriction: free of charge

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/46/2022-PPS.pdf
    Download Restriction: free of charge

    File URL: https://libkey.io/10.17221/46/2022-PPS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpps:v:58:y:2022:i:4:id:46-2022-pps. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.