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Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants

Author

Listed:
  • Pavel Cejnar

    (Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic)

  • Ludmila Ohnoutková

    (Department of Chemical Biology and Genetics, Centre of the Hana Region for Biotechnological and Agricultural Research, Palacky University Olomouc, Olomouc, Czech Republic)

  • Jan Ripl

    (Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic)

  • Jiban Kumar Kundu

    (Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic)

Abstract

We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.

Suggested Citation

  • Pavel Cejnar & Ludmila Ohnoutková & Jan Ripl & Jiban Kumar Kundu, 2019. "Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 55(2), pages 81-89.
    • Handle: RePEc:caa:jnlpps:v:55:y:2019:i:2:id:42-2018-pps
    DOI: 10.17221/42/2018-PPS
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    References listed on IDEAS

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    1. Josef Vacke & Radim Cibulka, 2000. "Comparison of DAS-ELISA and enzyme amplified ELISA for detection of Wheat dwarf virus in host plants and leafhopper vectors," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 36(2), pages 41-45.
    2. Josef Vacke & Radim Cibulka, 1999. "Silky bent grass (Apera spica-venti [L.] Beauv.) - a new host and reservoir of wheat dwarf virus," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 35(2), pages 47-50.
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