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Genetic diversity of the Cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detection

Author

Listed:
  • Beata Hasiów-Jaroszewska
  • Daria Budzyńska

    (Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland)

  • Natasza Borodynko-Filas

    (Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland)

Abstract

To analyse the genetic diversity of the Cucumber green mottle mosaic virus (CGMMV) population in Poland and to establish the phylogenetic relationships between the Polish and other isolates described to date, 91 isolates were collected from cucumber plants. The analysis, based on coat protein (CP) gene, revealed the presence of two phylogenetic groups: one consisting of the Polish isolates collected in 2017 and those originated mainly from Asia region and the second including the Polish isolates collected in 2016 and the others from European countries. The sensitive, specific, and rapid one-step loop-mediated isothermal amplification assay was developed for the early detection of genetically diverse CGMMV isolates in seeds and plant material.

Suggested Citation

  • Beata Hasiów-Jaroszewska & Daria Budzyńska & Natasza Borodynko-Filas, 2019. "Genetic diversity of the Cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detection," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 55(1), pages 1-7.
    • Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:92-2018-pps
    DOI: 10.17221/92/2018-PPS
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