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Cloning and characterisation of nanobodies against the coat protein of Zucchini yellow mosaic virus

Author

Listed:
  • Adel M. Zakri
  • Abdullah A. Al-Doss

    (Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food &)

  • Markus Sack

    (Agriculture Science, King Saud University, Riyadh, Saudi Arabia)

  • Ahmed A. Ali

    (Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food &)

  • Emad M. Samara

    (Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany)

  • Basem S. Ahmed

    (Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food &)

  • Mahmoud. A. Amer
  • Omar. A. Abdalla
  • Mohammed A. Al-Saleh

Abstract

Zucchini yellow mosaic virus (ZYMV), in the family Potyviridae, causes an economically important disease. Antibodies are valuable reagents for diagnostic assays to rapidly detect viral infection. Here, we report the isolation of camel-derived variable domains of the heavy chain antibody (VHH, also called nanobodies) directed against the coat protein (CP) of ZYMV. Several nanobodies that specifically recognise ZYMV-CP were identified. The isolated nanobodies showed binding not only to recombinant ZYMV-CP but also to native ZYMV, indicating that these nanobodies can be used in diagnostic tools to detect viral infections.

Suggested Citation

  • Adel M. Zakri & Abdullah A. Al-Doss & Markus Sack & Ahmed A. Ali & Emad M. Samara & Basem S. Ahmed & Mahmoud. A. Amer & Omar. A. Abdalla & Mohammed A. Al-Saleh, 2018. "Cloning and characterisation of nanobodies against the coat protein of Zucchini yellow mosaic virus," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 54(4), pages 215-221.
    • Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:158-2017-pps
    DOI: 10.17221/158/2017-PPS
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