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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation

Author

Listed:
  • Antonios Zambounis

    (Laboratory of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece)

  • Anastasios Samaras

    (Laboratory of Phytopathology, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece)

  • Aliki Xanthopoulou

    (Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki, Greece)

  • Maslin Osathanunkul

    (Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand)

  • Leonardo Schena

    (Departement of Agricultural Science, Faculty of Agricultural Science, Mediterranea University of Reggio Calabria, Reggio Calabria, Italy)

  • Athanasios Tsaftaris

    (Laboratory of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece
    Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki, Greece)

  • Panagiotis Madesis

    (Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki, Greece)

Abstract

A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles. The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype, revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker was sufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing the identification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducing the risk of contamination.

Suggested Citation

  • Antonios Zambounis & Anastasios Samaras & Aliki Xanthopoulou & Maslin Osathanunkul & Leonardo Schena & Athanasios Tsaftaris & Panagiotis Madesis, 2016. "Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 52(3), pages 176-181.
    • Handle: RePEc:caa:jnlpps:v:52:y:2016:i:3:id:179-2015-pps
    DOI: 10.17221/179/2015-PPS
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    Cited by:

    1. Antonios Zambounis & Eleni Stefanidou & Panagiotis Madesis & Jovana Hrustić & Milica Mihajlović & Brankica Tanović, 2021. "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 57(1), pages 38-46.

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