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Real-time PCR applied to study on plant pathogens: potential applications in diagnosis - a review

Author

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  • Seyed Mahyar MIRMAJLESSI

    (Department of Field Crops and Grassland Husbandry and)

  • Evelin LOIT

    (Department of Field Crops and Grassland Husbandry and)

  • Marika MÄND

    (Department of Plant Protection, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia)

  • Seyed Mojtaba MANSOURIPOUR

    (Department of Plant Pathology, North Dakota State University, Fargo, USA$2)

Abstract

Quantitative real-time PCR (qPCR) technique incorporates traditional polymerase chain reaction (PCR) efficiency with the production of a specific fluorescent signal, measuring the kinetics of the reaction in the early PCR phases and providing quantification of specific targets in various environmental samples. There are an increasing number of chemistries to detect PCR products, which are widely used in plant pathology as they cluster into the amplicon sequence non-specific and sequence-specific techniques. In this review, we illustrate a general description of major chemistries and discuss some considerations for assay development as it applies for a wide range of applications in epidemiological studies. The technique has become the gold standard for early detection of pathogens and a fundamental tool in the research laboratory.

Suggested Citation

  • Seyed Mahyar MIRMAJLESSI & Evelin LOIT & Marika MÄND & Seyed Mojtaba MANSOURIPOUR, 2015. "Real-time PCR applied to study on plant pathogens: potential applications in diagnosis - a review," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 51(4), pages 177-190.
    • Handle: RePEc:caa:jnlpps:v:51:y:2015:i:4:id:104-2014-pps
    DOI: 10.17221/104/2014-PPS
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    References listed on IDEAS

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    1. Miloslav Zouhar & Jana Mazáková & Evženie Prokinová & Marie Váňová & Pavel Ryšánek, 2010. "Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 46(3), pages 107-115.
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