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Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Author

Listed:
  • Tara SOLGI

    (National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran)

  • Mehdi MORADYAR

    (National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran)

  • Mohammad Reza ZAMANI

    (National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran)

  • Mostafa MOTALLEBI

    (National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran)

Abstract

An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum. Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum were significantly retarded when compared to non-transgenic canola plant.

Suggested Citation

  • Tara SOLGI & Mehdi MORADYAR & Mohammad Reza ZAMANI & Mostafa MOTALLEBI, 2015. "Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 51(1), pages 6-12.
    • Handle: RePEc:caa:jnlpps:v:51:y:2015:i:1:id:83-2013-pps
    DOI: 10.17221/83/2013-PPS
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