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Distribution of Plum pox virus strains in natural sources in the Czech Republic

Author

Listed:
  • Jaroslav Polák

    (Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Petr Komínek

    (Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

The distribution of Plum pox virus (PPV) strains, PPV-D, PPV-M and PPV-Rec, was investigated in the Czech Republic in 2005-2008. Fifty-two to ninety-four samples of flowers or leaves of plum, myrobalan and blackthorn trees from different regions were tested in individual years. The presence of PPV was detected by DAS-ELISA with serotype-specific polyclonal antibodies. PPV-M was proved by DASI-ELISA with serotype-specific monoclonal antibodies; PPV-D, PPV-M and PPV-Rec were detected by RT-PCR in leaf samples from PPV infected trees. The presence of PPV-D ranged from 94.6% to 100%, the presence of PPV-M from 0.0% to 2.3% and the presence of PPV-Rec from 0.0% to 3.1% during 2005-2008. More than 95% of analysed samples of PPV were infected with PPV-D and less than 2.5% of analysed samples of PPV were infected with PPV-M or PPV-Rec. The presence of PPV-C was not proved in sweet cherry and sour cherry trees. The presence of PPV-EA was not proved in apricot trees.

Suggested Citation

  • Jaroslav Polák & Petr Komínek, 2009. "Distribution of Plum pox virus strains in natural sources in the Czech Republic," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 45(4), pages 144-147.
    • Handle: RePEc:caa:jnlpps:v:45:y:2009:i:4:id:31-2009-pps
    DOI: 10.17221/31/2009-PPS
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