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Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR

Author

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  • Shesh Kumari

    (Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

A one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The protocol was used to test infected screen house plants and also plants from orchards and vineyards where the vector (Xiphinema diversicaudatum) of SLRSV was detected from the soil. The one-step RT-PCR protocol is rapid and sensitive and has the potential to be used for the diagnosis of CLRV and SLRSV in routine diagnostic laboratories.

Suggested Citation

  • Shesh Kumari, 2009. "Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 45(4), pages 140-143.
    • Handle: RePEc:caa:jnlpps:v:45:y:2009:i:4:id:3-2009-pps
    DOI: 10.17221/3/2009-PPS
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    References listed on IDEAS

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    1. Jaroslav Polák & Michaela Chaloupková & Milan Jokeš, 2004. "Biological and serological procedures to detect three nepoviruses in fruit trees," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 40(4), pages 121-127.
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