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In vitro storage of plum germplasm by slow growth

Author

Listed:
  • S. Gianní

    (Department of Agricultural and Forest Sciences, University of Palermo, Palermo, Italy)

  • F. Sottile

    (Department of Agricultural and Forest Sciences, University of Palermo, Palermo, Italy)

Abstract

In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. - two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage.

Suggested Citation

  • S. Gianní & F. Sottile, 2015. "In vitro storage of plum germplasm by slow growth," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 42(2), pages 61-69.
    • Handle: RePEc:caa:jnlhor:v:42:y:2015:i:2:id:186-2014-hortsci
    DOI: 10.17221/186/2014-HORTSCI
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    Cited by:

    1. Francesco Sottile & Nicole Roberta Giuggioli & Daniela Torello Marinoni & Cristiana Peano & Maria Beatrice Del Signore, 2020. "Selection and micropropagation of valuable caper genotypes," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 47(2), pages 110-116.

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