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Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure

Author

Listed:
  • Dj. Ružić

    (Fruit Research Institute, Čačak, Serbia)

  • T. Vujović

    (Fruit Research Institute, Čačak, Serbia)

  • R. Cerović

    (Innovation Center, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia)

Abstract

In vitro-grown shoot tips of Gisela 5 (Prunus cerasus × Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23°C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0°C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30, 40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.

Suggested Citation

  • Dj. Ružić & T. Vujović & R. Cerović, 2014. "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 41(2), pages 55-63.
    • Handle: RePEc:caa:jnlhor:v:41:y:2014:i:2:id:234-2013-hortsci
    DOI: 10.17221/234/2013-HORTSCI
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    References listed on IDEAS

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    1. T. Vujović & Dj. Ružić & R. Cerović, 2012. "In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 39(3), pages 101-107.
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    Cited by:

    1. Bożena Pawłowska & Jarmila Neugebauerová & Monika Bieniasz & Bożena Szewczyk-Taranek & Ivo Ondrasek, 2019. "Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potential monitored in four-year-old regenerants," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 46(2), pages 90-97.
    2. Alois Bilavčík & Jiří Zámečník & Miloš Faltus & Petra Jadrná, 2019. "Analysis of freezable water content by DSC for apple dormant bud cryopreservation," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 46(4), pages 163-170.

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