IDEAS home Printed from https://ideas.repec.org/a/caa/jnlhor/v39y2012i1id70-2011-hortsci.html
   My bibliography  Save this article

RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis

Author

Listed:
  • Y. Ii

    (Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan)

  • A. Uragami

    (National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Tsukuba, Japan)

  • Y. Uno

    (Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan)

  • M. Kanechi

    (Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan)

  • N. Inagaki

    (Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan)

Abstract

Asparagus (Asparagus officinalis L.) plants are dioecious. All-male cultivars are desired because of their higher yields. To increase the proportion of male individuals planted in the field and expedite the breeding of all-male cultivars in asparagus, development of generally applicable molecular markers to distinguish male and female individuals is required. Bulked genomic DNA samples from ten male (XY) and ten female (XX) plants was screened with 10-bp random primers. Of the 188 primers tested, the primer T35R54 produced a 1600-bp fragment observed only in male individuals. The specificity of this T35R54-1600 marker was verified using DNA from one supermale (YY) and one female (XX) breeding line and their four F1 progenies (XY). The T35R54-1600 marker fragment was observed in both supermale and all-male lines. The sequence of the T35R54 primer (5'-TTCACGGTGG-3') was absent among the sequences of primers or amplified fragments from previous studies. Therefore, this marker could be useful as a sex-related marker in future studies to increase the reliability of sex determination in asparagus.

Suggested Citation

  • Y. Ii & A. Uragami & Y. Uno & M. Kanechi & N. Inagaki, 2012. "RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 39(1), pages 33-37.
    • Handle: RePEc:caa:jnlhor:v:39:y:2012:i:1:id:70-2011-hortsci
    DOI: 10.17221/70/2011-HORTSCI
    as

    Download full text from publisher

    File URL: http://hortsci.agriculturejournals.cz/doi/10.17221/70/2011-HORTSCI.html
    Download Restriction: free of charge
    File URL: http://hortsci.agriculturejournals.cz/doi/10.17221/70/2011-HORTSCI.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/70/2011-HORTSCI?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlhor:v:39:y:2012:i:1:id:70-2011-hortsci. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.