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Biotechnological methods utilized in Cucumis research - A review

Author

Listed:
  • B. Navrátilová

    (Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic)

  • D. Skálová

    (Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic)

  • V. Ondřej

    (Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic)

  • M. Kitner

    (Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic)

  • A. Lebeda

    (Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic)

Abstract

Our biotechnological research on selected Cucumis species has encompassed interspecific hybridization via embryo-rescue, in vitro pollination, somatic hybridization and cytogenetics of protoplasts. Embryo-rescue and in vitro pollination are suitable in vitro techniques for production of hybrid embryos. These methods were tested and optimized for cucurbits. Protoplast culture is another valuable tool for biotechnological applications, such as somatic hybridization and genetic transformation. We study protoplast dedifferentiation not only as a biotechnological application in breeding systems, but mainly to describe mechanisms of obtaining totipotency. Protoplasts of cucurbits were studied cytogenetically to observe changes in nuclear architecture during protoplastization and regeneration and for comparison with the expression profile obtained using cDNA-AFLP techniques and reverse transcription for the specific genes involved.

Suggested Citation

  • B. Navrátilová & D. Skálová & V. Ondřej & M. Kitner & A. Lebeda, 2011. "Biotechnological methods utilized in Cucumis research - A review," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 38(4), pages 150-158.
    • Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:143-2010-hortsci
    DOI: 10.17221/143/2010-HORTSCI
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