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Influence of growth regulators and nitrogenous compounds on in vitro bulblet formation and growth in oriental lily

Author

Listed:
  • S. Kumar

    (Department of Biotechnology, University of Horticulture & Forestry, Solan, India)

  • V. Awasthi

    (Department of Biotechnology, University of Horticulture & Forestry, Solan, India)

  • J. K. Kanwar

    (Department of Biotechnology, University of Horticulture & Forestry, Solan, India)

Abstract

The influence of growth regulators and nitrogenous compounds on in vitro bulblet formation and growth was studied in two hybrids of Lilium. Bulbscales isolated from pre-cooled bulbs of hybrids Rosato and Marco Polo were used. The basal portion with plate (5 × 6 mm) of inner bulbscales was cultured on Murashige and Skoog (MS) medium containing 0.5 or 1 mg/dm3 naphthaleneacetic acid (NAA) and/or benzyladenine (BA). The presence of NAA (0.5 mg per dm3) showed higher explant regeneration, producing about three bulblets per explant as compared to control. About four bulblets per explant were produced at both concentrations of BA. The bulblets with significantly higher fresh weight were obtained on medium containing NAA. Approximately a three-fold increase of bulblet fresh weight was observed with all the concentrations of TDZ in both cultivars. The bulblets cultured with nitrogenous compounds after attaining the size of 14-16 cm flowered during the second year of the growing period without any phenotypic variations.

Suggested Citation

  • S. Kumar & V. Awasthi & J. K. Kanwar, 2007. "Influence of growth regulators and nitrogenous compounds on in vitro bulblet formation and growth in oriental lily," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 34(2), pages 77-83.
    • Handle: RePEc:caa:jnlhor:v:34:y:2007:i:2:id:1853-hortsci
    DOI: 10.17221/1853-HORTSCI
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    Cited by:

    1. R. Kapoor & S. Kumar & J. K. Kanwar, 2008. "Bulblet regeneration from ex vitro root explant in lily hybrids," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 35(3), pages 107-112.

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