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Identification of sucrase activity in cell suspension and culture medium of melon

Author

Listed:
  • J. Stano

    (Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic)

  • K. Mičieta

    (Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic)

  • A. Barth

    (Institute of Biochemistry, M. Luther University, Halle/Saale, Federal Republic of Germany)

  • M. Valšíková

    (Research Institute of Vegetables, Nové Zámky, Slovak Republic)

  • M. Fulmeková

    (Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic)

  • P. Matejka

    (Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic)

  • M. Varadínová

    (Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic)

Abstract

The activity of (soluble acid) sucrase was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple and rapid procedure for the identification and determination of extracellular sucrase from a culture medium of watermelon cell suspension cultures is described. Sucrose was used as a substrate for the determination of extracellular and intracellular activities of the enzyme. Intracellular activity was estimated from the cell suspension. The results show a 91.5-92.0% intracellular and 8.0-8.5% extracellular distribution of sucrase activity. The described method enables to carry out a rapid, simple and specific detection of extracellular sucrase in plants.

Suggested Citation

  • J. Stano & K. Mičieta & A. Barth & M. Valšíková & M. Fulmeková & P. Matejka & M. Varadínová, 2005. "Identification of sucrase activity in cell suspension and culture medium of melon," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 32(3), pages 104-107.
    • Handle: RePEc:caa:jnlhor:v:32:y:2005:i:3:id:3774-hortsci
    DOI: 10.17221/3774-HORTSCI
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