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Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs

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  • Wen-Kui Sun

    (Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China
    Chengdu Medical College, School of Laboratory Medicine, Chengdu, P.R. China)

  • Chi Cheng

    (College of Chemistry and Life Science, Chengdu Normal University, Chengdu, P.R. China)

  • Rui Liu

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Yi-Hui Chen

    (Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China)

  • Kai Zeng

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Xiao-Hui Chen

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Yi-Ren Gu

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Jiang-Ling Li

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Xue-Bin Lv

    (Sichuan Academy of Animal Science, Chengdu, P.R. China)

  • Rong Gao

    (Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China)

Abstract

The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin receptor (GHSR), LEP, leptin receptor (LEPR), SST and CCK) by reverse transcription-qPCR (RT-qPCR). TP generally had higher mRNA expressions of GHSR, LEP, LEPR, SST and CCK genes compared to YP, and expressed lower levels of the GHRL gene in most tissues of the digestive tract. In both breeds, plasma levels of the expressed proteins were more closely correlated with the feed intake and growth than with mRNA levels of the target genes. Our data indicate that TP possess special gene expression patterns in the gastrointestinal tract compared to YP, which is consistent with its unique feed intake and adaptation to harsh environment.

Suggested Citation

  • Wen-Kui Sun & Chi Cheng & Rui Liu & Yi-Hui Chen & Kai Zeng & Xiao-Hui Chen & Yi-Ren Gu & Jiang-Ling Li & Xue-Bin Lv & Rong Gao, 2019. "Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(6), pages 239-247.
    • Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:40-2018-cjas
    DOI: 10.17221/40/2018-CJAS
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    1. Mingzhou Li & Honglong Wu & Zonggang Luo & Yudong Xia & Jiuqiang Guan & Tao Wang & Yiren Gu & Lei Chen & Kai Zhang & Jideng Ma & Yingkai Liu & Zhijun Zhong & Jing Nie & Shuling Zhou & Zhiping Mu & Xia, 2012. "An atlas of DNA methylomes in porcine adipose and muscle tissues," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
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