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Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

Author

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  • Zuzana Biniová

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
    NATURAL spol. s r.o., Hradištko pod Medníkem, Czech Republic)

  • Luděk Stádník

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Martina Doležalová

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Jaromír Ducháček

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

Abstract

The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed.

Suggested Citation

  • Zuzana Biniová & Luděk Stádník & Martina Doležalová & Jaromír Ducháček, 2018. "Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(10), pages 399-407.
    • Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:10:id:117-2018-cjas
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    References listed on IDEAS

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    1. Tomáš Slanina & Michal Miškeje & Filip Tirpák & Martyna Błaszczyk & Robert Stawarz & Peter Massányi, 2018. "Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(4), pages 127-135.
    2. M. Doležalová & L. Stádník & Z. Biniová & J. Ducháček & R. Stupka, 2016. "Equilibration and freezing interactions affecting bull sperm characteristics after thawing," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(11), pages 515-525.
    3. Youwei Li & Mingju Sun & Jiang Zhu & Guangzhong Jiao & Juan Lin & Haibo Dong & Jinghe Tan & Jiabo Zhou, 2017. "Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(12), pages 519-526.
    4. P. Padrik & T. Hallap & T. Kaart & T. Bulitko & Ü. Jaakma, 2012. "Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(10), pages 490-497.
    5. Jiří Šichtař & Anežka Nehasilová & Ondřej Šimoník & Filipa Bubeníčková, 2017. "Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions - A pilot study," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(6), pages 227-233.
    6. Eva Tvrdá & Hana Greifová & Alica Mackovich & Faridullah Hashim & Norbert Lukáč, 2018. "Curcumin offers antioxidant protection to cryopreserved bovine semen," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(7), pages 247-255.
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