IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v62y2017i7id93-2015-cjas.html
   My bibliography  Save this article

Identification and characterization of bovine mammary peptide transporters in response to tripeptide and lactogenic hormone treatment

Author

Listed:
  • Yan Cui

    (Department of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Xingfu Zhang

    (Institute of Animal Nutrition, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China)

  • Chunli Guo

    (Department of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Ruiping Du

    (Institute of Animal Nutrition, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China)

  • Gaowa Ailun

    (Department of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Changjin Ao

    (Department of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Min Gao

    (Institute of Animal Nutrition, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China)

Abstract

Oligopeptide transportation is mediated by the peptide transporter (PepT), which consists of two isoforms, PepT1 and PepT2. Because PepT play essential roles in amino acid metabolism and cell growth, the aim of the present study was to identify these transporters in bovine mammary glands and to analyze the potential functions of these transporters in mammary epithelial cells. Abundance of PepT1 and PepT2 mRNA was successfully measured in both mammary glands and cultured mammary epithelial cells. In addition, the two proteins were examined using immunohistochemistry, immunocytochemistry, and Western blots. The response of mammary epithelial cells to tripeptide and lactogenic hormone treatment was assayed. The PepT mRNA abundance of cultured epithelial cells and secreted protein in the culture medium were increased after tri-peptide substitution and addition of hormones such as insulin, hydrocortisone, and prolactin. The response of mammary epithelial cells to tripeptide and hormone treatments suggests that PepT affects the mammary gland function and increases bovine milk production.

Suggested Citation

  • Yan Cui & Xingfu Zhang & Chunli Guo & Ruiping Du & Gaowa Ailun & Changjin Ao & Min Gao, 2017. "Identification and characterization of bovine mammary peptide transporters in response to tripeptide and lactogenic hormone treatment," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(7), pages 296-305.
    • Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:7:id:93-2015-cjas
    DOI: 10.17221/93/2015-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/93/2015-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/93/2015-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/93/2015-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Keywords

    bovine mammary gland; PepT1; RT-PCR; lactogen;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:62:y:2017:i:7:id:93-2015-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.