IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v61y2016i5id34-2015-cjas.html
   My bibliography  Save this article

Genetic variation, association analysis, and expression pattern of SMAD3 gene in Chinese cattle

Author

Listed:
  • T. Shi

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • Y. Xu

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • M.-J. Yang

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • Y. Zhou

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • M. Liu

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • X.-Y. Lan

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • C.-Z. Lei

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

  • X.-L. Qi

    (Bureau of Animal Husbandry of Biyang County, Biyang, P.R. China)

  • F.-P. Lin

    (Bureau of Animal Husbandry of Biyang County, Biyang, P.R. China)

  • Y.-Y. Bai

    (Animal Health Supervision in Henan Province, Zhengzhou, P.R. China)

  • H. Chen

    (Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China)

Abstract

SMAD3, a member of SMAD transcription factors, plays a key role in transforming growth factor-beta (TGF-β) signalling pathway and regulation of muscle growth. However, there was no strong evidence of association between SMAD3 polymorphisms and body traits in animals. In this study, single nucleotide polymorphisms (SNPs) in SMAD3 gene were detected in four Chinese cattle breeds (Qinchuan, Jiaxian, Nanyang, and Caoyuan) by using DNA pool sequencing and PCR-RFLP, and their effects on gene expression and growth traits were evaluated in Qinchuan cattle. The results showed that four novel SNPs (NC_007308.5 c.-2017A>G, g.101664C>G, g.105829A>G, and g.114523A>G) in promoter, intron 3, and intron 5 were found in four cattle breeds. NC_007308.5 c.-2017A>G and g.114523A>G were significantly (P < 0.05) associated with SMAD3 gene expression. Furthermore, the four SNPs were strikingly (P < 0.05) associated with rump length, chest girth, and body weight in two-year-old Qinchuan cattle. Our results provided the evidence that SNPs in SMAD3 were associated with cattle traits, which showed the possibility that the four SNPs could be novel molecular markers for beef cattle breeding and genetics.

Suggested Citation

  • T. Shi & Y. Xu & M.-J. Yang & Y. Zhou & M. Liu & X.-Y. Lan & C.-Z. Lei & X.-L. Qi & F.-P. Lin & Y.-Y. Bai & H. Chen, 2016. "Genetic variation, association analysis, and expression pattern of SMAD3 gene in Chinese cattle," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(5), pages 209-216.
    • Handle: RePEc:caa:jnlcjs:v:61:y:2016:i:5:id:34-2015-cjas
    DOI: 10.17221/34/2015-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/34/2015-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/34/2015-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/34/2015-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jindřich Čítek & Lenka Hanusová & Michaela Brzáková & Libor Večerek & Lothar Panicke & Lucie Lískovcová, 2018. "Associations between gene polymorphisms, breeding values, and glucose tolerance test parameters in German Holstein sires," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(5), pages 167-173.

    More about this item

    Keywords

    SMAD3 polymorphisms; mRNA level; body traits; bovine;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:61:y:2016:i:5:id:34-2015-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.