IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v61y2016i1id8662-cjas.html
   My bibliography  Save this article

Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition

Author

Listed:
  • M. Alomar

    (Division of Animal Production, Department of Agriculture, Atomic Energy Commission of Syria, Damascus, Syria)

  • M. Alzoabi

    (Division of Animal Production, Department of Agriculture, Atomic Energy Commission of Syria, Damascus, Syria)

  • M. Zarkawi

    (Division of Animal Production, Department of Agriculture, Atomic Energy Commission of Syria, Damascus, Syria)

Abstract

The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of H2O2 production from both live and dead spz at 1 × 106, 3 × 106, and6 × 106 spz/well concentrations were assessed in the tyrode albumin lactate (TAL) medium every 15 min for 120 min. An increase in H2O2 production from both live and dead spz was noted with a significant difference (P < 0.05) between the 1 × 106 and 6 × 106 spz/well concentrations. Although dead sperm generated higher amounts of H2O2 than live ones, no significant differences (P > 0.05) were observed between the two types of sperm for the three different concentrations. The generation of H2O2 by ram spz was also compared in the presence and absence of nicotinamide adenine dinucleotide phosphate (NADPH) and phenylalanine, substrates of the two specific oxidases. The supplementation with these substrates significantly (P < 0.05) increased the amounts of H2O2 generated from both live and dead spz, but for the two substrates, the increase was higher with dead than with live spz especially when phenylalanine was added. Addition of the antioxidant catalase significantly (P < 0.05) decreased the generation of H2O2 by live and dead spz with no significant differences (P > 0.05) between the two types of sperm before or after the antioxidant addition. This study showed the ability of live and dead ram spz to generate H2O2 in TAL medium. This ability was significantly influenced by the addition of NADPH and phenylalanine and also by the supplementation of the antioxidant catalase.

Suggested Citation

  • M. Alomar & M. Alzoabi & M. Zarkawi, 2016. "Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(1), pages 1-7.
    • Handle: RePEc:caa:jnlcjs:v:61:y:2016:i:1:id:8662-cjas
    DOI: 10.17221/8662-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/8662-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/8662-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/8662-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:61:y:2016:i:1:id:8662-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.