IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v59y2014i7id7530-cjas.html
   My bibliography  Save this article

Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

Author

Listed:
  • I. Lebeda

    (South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic)

  • I. Gazo

    (South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic)

  • M. Flajshans

    (South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic)

Abstract

Chromosomal manipulations in sturgeons, particularly gynogenesis, are interesting due to the potential to change female ratio in progeny that can be useful for caviar production. The optimization of UV treatment for induction of gynogenesis is complicated due to high and variable optical density of the milt due to differential spermatozoa concentration, and because of sensitivity of spermatozoa's motility apparatus. Therefore in this study we compared chemical methods of sperm treatment as an alternative to short wave-length UV treatment; evaluation considers impact on spermatozoa motility, DNA integrity, and efficiency of DNA inactivation. Dimethyl sulfate (DMS) in concentrations of 2.5-30mM was applied to spermatozoa in order to inactivate DNA. Also ethidium bromide (EB), psoralen (PS), and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) were used to increase sensitivity of spermatozoa's DNA to long wavelength UV-A light (360 nm). CASA analyses of treated sperm showed strong negative effects on spermatozoa motility with the increasing concentration of active substances. Additionally in case of PS, EB, and DMS treatment comet assay did not reveal significant DNA damage of sperm at the range of concentrations relatively safe for spermatozoa motility. Flow cytometric analysis of relative DNA content in larvae resulting from activation of normal ova of sterlet with the treated sperm showed low efficiency of haploid gynogenesis induction. The putative gynogenetic larvae were found after treatment with PS in concentrations higher than 18µM and EB higher than 10µM followed by UV-A irradiation at the dose of 900 J/m2 and DMS up to 5mM. Because of an overwhelming impact on the sperm motility and relatively low DNA damage, treatment of sperm with PS, EB or DMS did not prove efficient compared with a widely used UV-C irradiation treatment. In contrast, treatment with AMT followed by UV-A showed lower influence on spermatozoa motility and higher efficiency of DNA damaging resulting in the higher percentage of gynogenotes in the progeny, thus could be considered as a possible substitution for UV-C treatment.

Suggested Citation

  • I. Lebeda & I. Gazo & M. Flajshans, 2014. "Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(7), pages 310-326.
    • Handle: RePEc:caa:jnlcjs:v:59:y:2014:i:7:id:7530-cjas
    DOI: 10.17221/7530-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/7530-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/7530-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/7530-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:59:y:2014:i:7:id:7530-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.