IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v59y2014i6id7497-cjas.html
   My bibliography  Save this article

Effect of Lactobacillus plantarum LS/07 in combination with flaxseed oil on the microflora, enzymatic activity, and histological changes in the development of chemically induced precancerous growth in the rat colon

Author

Listed:
  • L. Strojný

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • J. Štofilová

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • E. Hijová

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • V. Szabadosová

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • R. Salaj

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • I. Bertková

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • A. Chmelárová

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • D. Čokášová

    (Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University Olomouc, Olomouc, Czech Republic)

  • B. Pramuková

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • A. Brandeburová

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • A. Bomba

    (Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • N. Bobrov

    (Department of Forensic Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

  • P. Suchánek

    (Department of Gynaecology and Obstetrics, Faculty of Medicine, P.J. Šafárik University in Košice, Košice, Slovak Republic)

Abstract

This experimental study showed the effect of L. plantarum LS/07 on the changes in total counts of coliform bacteria, enzymatic activities, cytokine and histological changes in the colonic mucosa, chemically induced by the application of 1.2-dimethylhydrazine dihydrochloride (1.2-DMH) in rats once a week during five weeks in the positive control group, probioticum (PRO) group, and probioticum and oil (PRO-O) group. A total of 32 rats were randomly assigned to four treatment groups: negative control group (diet without any supplements), positive control group (1.2-DMH without any supplements), PRO group (L. plantarum LS/07 at the dose (average concentration 2.88 × 109 CFU/ml) + 1.2-DMH), PRO-O group (L. plantarum LS/07 + flaxseed oil (40 g/kg feed) + 1.2-DMH). All rats were kept on fat diet (35 g/kg). The results showed a significant (P < 0.05) decrease in total counts of coliform bacteria in PRO-O group. Results of enzymatic activities of isolated strain showed that L. plantarum LS/07 does not produce enzyme β-glucuronidase. Significantly higher activity of β-galactosidase was in the groups PRO (P < 0.001) and PRO-O (P < 0.01). On the other hand, significantly lower (P < 0.001) enzymatic activity of ß-glucuronidase and β-glucosidase was found in PRO-O group compared with positive control group. The cytokine activity of tumor necrosis factor (TNFα) was significantly decreased in groups PRO (P < 0.001) and PRO-O (P < 0.01) compared to the positive control group. The histological examination showed different changes (aberrant crypts and goblet cells) in experimental groups in comparison to the negative control group. Our study indicates that L. plantarum LS/07 in combination with flaxseed oil had a positive influence on colon microflora, enzymatic activities, and cytokine activity. The total results showed that potentiating effects of natural components were markedly achieved in the experimental group PRO-O.

Suggested Citation

  • L. Strojný & J. Štofilová & E. Hijová & V. Szabadosová & R. Salaj & I. Bertková & A. Chmelárová & D. Čokášová & B. Pramuková & A. Brandeburová & A. Bomba & N. Bobrov & P. Suchánek, 2014. "Effect of Lactobacillus plantarum LS/07 in combination with flaxseed oil on the microflora, enzymatic activity, and histological changes in the development of chemically induced precancerous growth in," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(6), pages 268-277.
    • Handle: RePEc:caa:jnlcjs:v:59:y:2014:i:6:id:7497-cjas
    DOI: 10.17221/7497-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/7497-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/7497-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/7497-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. R. Kasteľ & A. Bomba & L. Vasko & A. Trebunova & P. Mach, 2007. "The effect of probiotics potentiated with polyunsaturated fatty acids on the digestive tract of germ-free piglets," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(2), pages 63-68.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. V. Szabadosova & E. Hijova & L. Strojny & R. Salaj & A. Bomba & D. Cokasova & B. Pramukova & J. Zofcakova & I. Bertkova & A. Supukova & A. Soltesova & A. Brandeburova & A. Chmelarova, 2013. "Effect of horse chestnut and inulin as single supplements or in combination on chemically induced colon cancer in rats," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 58(9), pages 491-499.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:59:y:2014:i:6:id:7497-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.