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Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods

Author

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  • P. Homolka

    (Institute of Animal Science, Prague-Uhříněves, Czech Republic
    Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • V. Koukolová

    (Institute of Animal Science, Prague-Uhříněves, Czech Republic)

  • M. Podsedníček

    (Institute of Animal Science, Prague-Uhříněves, Czech Republic)

  • A. Hlaváčková

    (Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

Abstract

The aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods. Generally, maturation caused a significant increase in fibre fractions. With the increasing maturity of forage samples the in vivo, in vitro, and calculated in vivo (in vivocalcul) digestibilities of organic matter (OM) linearly decreased. The in vitro and in vivocalcul digestibilities of OM averaged 0.754 and 0.708 for red clover and 0.717 and 0.667 for lucerne, respectively. The in vivo OM digestibility averaged 0.710 for red clover and 0.666 for lucerne. Gross energy (GE), digestible energy (DE), metabolizable energy (ME), net energy for lactation (NEL), and net energy for growth (NEG) averaged 18.12, 12.41, 9.60, 5.67, 5.50 and 18.09, 11.56, 9.01, 5.26, 4.99 MJ/kg of dry matter for red clover and lucerne, respectively. The effect of a vegetative stage on energy values of both forages was diverged for various growth times. When data were pooled across the estimated season, seven cutting-specific equations for descriptions of GE, DE, ME, NEL, NEG, in vitro, and in vivo OM digestibilities were obtained for red clover and lucerne, separately. The red clover model expression gave similar prediction equations for lucerne. It was possible to predict cutting-specific equations with coefficients of determination R2 > 0.719 for red clover and R2 > 0.400 for lucerne of the variation in GE, DE, ME, NEL, and NEG. The in vitro and in vivo OM digestibility equations were predicted with R2 being 0.840 (in vitro) and 0.707 (in vivo) for red clover, and 0.979 (in vitro) and 0.937 (in vivo) for lucerne. The parameters of these specific equations were statistically preferable than the general model expression which included both forages together.

Suggested Citation

  • P. Homolka & V. Koukolová & M. Podsedníček & A. Hlaváčková, 2012. "Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(10), pages 454-468.
  • Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:10:id:6346-cjas
    DOI: 10.17221/6346-CJAS
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    References listed on IDEAS

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    1. B. Písaříková & J. Peterka & M. Trčková & J. Moudrý & Z. Zralý & I. Herzig, 2007. "The content of insoluble fibre and crude protein value of the aboveground biomass of Amaranthus cruentus and A. hypochondriacus," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(10), pages 348-353.
    2. V. Koukolová & P. Homolka & O. Koukol & F. Jančík, 2010. "Nutritive value of Trifolium pratense L. for ruminants estimated from in situ ruminal degradation of neutral detergent fibre and in vivo digestibility of organic matter and energy," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(9), pages 372-381.
    3. F. Jančík & V. Koukolová & P. Homolka, 2010. "Ruminal degradability of dry matter and neutral detergent fibre of grasses," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(9), pages 359-371.
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