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The ambiguity of freemartinism diagnosis in cattle revealed by cytogenetic and molecular techniques

Author

Listed:
  • J. Nowacka

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • M. Switonski

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • M. Mackowski

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • E. Slota

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • A. Radko

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • T. Zabek

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

  • K. Urbaniak

    (, M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U)

Abstract

Nineteen heifers and three male co-twins, originating from heterosexual twin pregnancies, were analysed with the use of cytogenetic and molecular techniques. A large number (50-400) of Giemsa stained metaphase spreads were studied and the proportion of XX and XY cell lines was calculated. The presence of two genes - SRY and AMEL (AMELX and AMELY) - was also analysed. Both approaches facilitated the identification of the XX/XY chimerism in 17 females and three males. The proportion of the co-twin cell line ranged from 1 to 99%. In two females no chimerism was detected: (1) 60,XY male chromosome complement in all 400 metaphase spreads; SRY-positive, AMELY-positive, AMELX-positive and (2) 60,XX female chromosome complement in all 200 metaphase spreads; SRY-negative, AMELY-negative, AMELX-positive. The usefulness of different techniques for the diagnosis of freemartinism is discussed.

Suggested Citation

  • J. Nowacka & M. Switonski & M. Mackowski & E. Slota & A. Radko & T. Zabek & K. Urbaniak, 2004. "The ambiguity of freemartinism diagnosis in cattle revealed by cytogenetic and molecular techniques," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(6), pages 239-243.
    • Handle: RePEc:caa:jnlcjs:v:49:y:2004:i:6:id:4306-cjas
    DOI: 10.17221/4306-CJAS
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