IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v55y2019i1id195-2017-cjgpb.html
   My bibliography  Save this article

The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)

Author

Listed:
  • Murat Aycan

    (Department of Field Crops, Graduate School of Natural and Applied Sciences, Ankara University, Diskapi, Ankara, Turkey)

  • Ramazan Beyaz

    (Department of Soil and Plant Nutrition, Faculty of Agriculture, Ahi Evran University, Bagbaşi, Kirşehir, Turkey)

  • Anzel Bahadir

    (Department of Biophysics, Faculty of Medicine, Duzce University, Konuralp, Duzce, Turkey)

  • Mustafa Yildiz

    (Department of Field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey)

Abstract

This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.

Suggested Citation

  • Murat Aycan & Ramazan Beyaz & Anzel Bahadir & Mustafa Yildiz, 2019. "The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 55(1), pages 20-27.
    • Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:1:id:195-2017-cjgpb
    DOI: 10.17221/195/2017-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/195/2017-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/195/2017-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/195/2017-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:55:y:2019:i:1:id:195-2017-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.