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Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markers

Author

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  • Josef PATZAK
  • Alena HENYCHOVÁ

    (Hop Research Institute Co., Ltd., Žatec, Czech Republic)

Abstract

Traditional hop (Humulus lupulus L.) cultivars have been used in the brewing industry for a long time. Globally, about ten new breeding lines were released to the market in each decade from ~1970 to 1999. Since 2006, the rate of release of new cultivars has increased tenfold. It is, therefore, important to identify their genotype and origin. Molecular genetic methods based on DNA are the most appropriate technology for this purpose. Recently, we developed an efficient marker system for the authenticity control of hop genotypes based on expressed sequence tag-simple sequence repeats (EST-SSR). In the present study, we enlarged the previously established EST-SSR set with 27 new polymorphic markers and evaluated molecular genetic variability within 135 traditional and new world hop cultivars. Two sets of 10 markers effectively differentiated all used cultivars, with the exception of cultivars derived from the same original genotype such as Saaz, Spalt, Tettnang and Nadwislawsky. Results of molecular genetic variability analyses corresponded with the genealogical and geographical origin of the key cultivars.

Suggested Citation

  • Josef PATZAK & Alena HENYCHOVÁ, 2018. "Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markers," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 54(2), pages 86-91.
    • Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:2:id:175-2016-cjgpb
    DOI: 10.17221/175/2016-CJGPB
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    Cited by:

    1. Pavel Donner & Jaroslav Pokorný & Josef Ježek & Karel Krofta & Josef Patzak & Josef Pulkrábek, 2020. "Influence of weather conditions, irrigation and plant age on yield and alpha-acids content of Czech hop (Humulus lupulus L.) cultivars," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 66(1), pages 41-46.

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