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Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia

Author

Listed:
  • Farnaz ABED-ASHTIANI

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

  • Jugah KADIR

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

  • Abbas NASEHI

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

  • Seyed-Reza HASHEMIAN-RAHAGHI

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

  • Ganesan VADAMALAI

    (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

  • Siti-Khadijah RAMBE

    (Laboratory of Bioresource Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)

Abstract

The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor, Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins.

Suggested Citation

  • Farnaz ABED-ASHTIANI & Jugah KADIR & Abbas NASEHI & Seyed-Reza HASHEMIAN-RAHAGHI & Ganesan VADAMALAI & Siti-Khadijah RAMBE, 2016. "Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 52(4), pages 145-156.
    • Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:102-2016-cjgpb
    DOI: 10.17221/102/2016-CJGPB
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