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Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus

Author

Listed:
  • Lin Lin
  • Chundan Zhang

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

  • Xinman Shao

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

  • Pupin Liu

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

  • Zhangfei Wu

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

  • Yanxia Lu

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

  • Hongzhen Guo

    (College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, China)

Abstract

Acetyl-coenzyme A (CoA) is a key metabolite produced by the acetyl-CoA synthetase (ACS) gene in energy metabolism and biosynthetic pathways. ACS is speculated to be the branching site of monacolin K (MK) and citrinin production and related to the metabolite production of Monascus. In this study, the ACS expression was inhibited by ribonucleic acid interference (RNAi). T7 was selected for a follow-up analysis of the lowest ACS expression, which was 0.401 times higher than that of the wild-type strain. The effects on the colony morphology of Monascus were determined. The morphological characteristics of mycelia and spores were observed under a scanning electron microscope. The contents of secondary metabolites, namely, MK and citrinin, were determined through high performance liquid chromatography (HPLC). Colour values were measured with a spectrophotometer. Results showed that the low ACS expression could inhibit the growth of Monascus colonies and the hypha and affect the formation and morphology of Monascus M1 spores. It could also inhibit the production of the main secondary metabolites, namely, MK, citrinin, and pigment.

Suggested Citation

  • Lin Lin & Chundan Zhang & Xinman Shao & Pupin Liu & Zhangfei Wu & Yanxia Lu & Hongzhen Guo, 2022. "Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 40(6), pages 414-421.
    • Handle: RePEc:caa:jnlcjf:v:40:y:2022:i:6:id:4-2022-cjfs
    DOI: 10.17221/4/2022-CJFS
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