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Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides

Author

Listed:
  • Xiang Hui Kong

    (Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
    Institute of Food Research, Hezhou University, Hezhou, China
    Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
    Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China)

  • Yin Peng Ma

    (Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
    Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China)

  • Yu Pan

    (Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China)

  • Wei Jiang

    (Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China)

  • Ding Jin Li

    (Institute of Food Research, Hezhou University, Hezhou, China)

  • Xi Jun Chen

    (Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
    Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China)

  • Guo Li Yang

    (Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
    Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China)

  • Zhen Hua Duan

    (Institute of Food Research, Hezhou University, Hezhou, China)

  • Jie Chi Zhang

    (Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
    Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China)

Abstract

Assessments of molecular weight distribution and activity/efficacy of Auricularia heimuer polysaccharides (AAP) are of substantial significance for its extraction process optimisation. In the present study, single-factor orthogonal test and response surface methodology were employed to optimise extraction conditions of AAP. Furthermore, a rat hyperlipidaemia model was established to compare the lipid-lowering activity of polysaccharides obtained by three extraction methods. Conditions for enzymatic hydrolysis were optimised as pH 5.0, 1% cellulase, 2.5% substrate concentration and enzymolysis time of 1.5 h, leading to an up to 31.8% polysaccharide yield and 89.13% of polysaccharides within the molecular weight range of 5 000 Da to 10 000 Da. The results of animal experiments showed that the lipid-lowering activity of enzymolysis-extracted polysaccharides was significantly higher than that of water- and ultrasonic-extracted ones (P < 0.01). So the present study revealed that enzymatic hydrolysis-extracted polysaccharides showed the strongest hypolipidaemia activity, providing a basis for the development of A. heimuer-based functional foods and drugs.

Suggested Citation

  • Xiang Hui Kong & Yin Peng Ma & Yu Pan & Wei Jiang & Ding Jin Li & Xi Jun Chen & Guo Li Yang & Zhen Hua Duan & Jie Chi Zhang, 2021. "Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 39(6), pages 452-459.
    • Handle: RePEc:caa:jnlcjf:v:39:y:2021:i:6:id:146-2020-cjfs
    DOI: 10.17221/146/2020-CJFS
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    References listed on IDEAS

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    1. Anet Režek Jambrak & Timothy J. Mason & Larysa Paniwnyk & Vesna Lelas, 2007. "Ultrasonic effect on pH, electric conductivity, and tissue surface of button mushrooms, Brussels sprouts and cauliflower," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 25(2), pages 90-99.
    2. Guoping Yu & Chonghui Yue & Xiaodan Zang & Chao Chen & Liangwei Dong & Yanqiu Liu, 2019. "Purification, characterization and in vitro bile salt-binding capacity of polysaccharides from Armillaria mellea mushroom," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 37(1), pages 51-56.
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