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Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis

Author

Listed:
  • Zhouyong Dong
  • Gang Tian

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

  • Zhaogang Xu

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

  • Mingyue Li

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

  • Min Xu

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

  • Yajun Zhou

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

  • Hui Ren

    (College of Food Science and Engineering, Jilin University, Changchun, P.R. China)

Abstract

The freshwater mussel protein was hydrolysed using ultrasound-assisted enzymolysis. Ultrasound-assisted freshwater mussel protein hydrolysates (UPH) were divided into four fractions (> 10, 6-10, 3-6, and < 3 kDa) using ultrafiltration, and the fraction with the highest antioxidant activity was further subdivided into four fractions (F1-F4) using gel chromatography. The amino acid compositions and antioxidant activities (DPPH, hydroxyl and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and inhibition of linoleic acid oxidation) of peptide fractions were investigated. The results showed that the antioxidant activity of the < 3 kDa fraction was significantly higher than that of UPH, > 10, 6-10, and 3-6 kDa fractions. The antioxidant activity of F2 was again higher compared with the < 3 kDa fraction and higher than that of F1, F3, and F4. Amino acid analysis showed that the antioxidant activities (except for chelating activity) of peptides increased with increasing hydrophobic amino acid content. The < 3 kDa and F2 fractions exhibited strong inhibition of linoleic acid oxidation, their effects being even better than that of ascorbic acid (Vc) and l-glutathione (GSH). Therefore, these peptide fractions from freshwater mussel may be a potential natural antioxidant that could be added to various foods.

Suggested Citation

  • Zhouyong Dong & Gang Tian & Zhaogang Xu & Mingyue Li & Min Xu & Yajun Zhou & Hui Ren, 2017. "Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 35(4), pages 328-338.
    • Handle: RePEc:caa:jnlcjf:v:35:y:2017:i:4:id:421-2016-cjfs
    DOI: 10.17221/421/2016-CJFS
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