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Detection of PCR inhibition in food and feed with a synthetic plasmid

Author

Listed:
  • Tereza Sovová
  • Barbora Křížová

    (Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic)

  • Lenka Drábková

    (Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic)

  • Jaroslava Ovesná

    (Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic)

Abstract

We present a successful use of the plasmid inhibition detection and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two meat meal samples and two samples made of cranberries (jam and dried fruit). The quantitative real-time polymerase chain reaction (qPCR) can be inhibited by various substances and the DNA content in the sample can be underestimated. It is necessary to identify the PCR inhibition and choose an optimal DNA isolation protocol to correctly evaluate the sample. In a previous study, we have developed an assay using plasmid DNA carrying a non-homologous random sequence identifying possible inhibitors in qPCR in food/feed samples. The plasmid assay allowed to effectively reveal the PCR inhibition in all of the different sample matrices and to choose an optimal DNA isolation protocol.

Suggested Citation

  • Tereza Sovová & Barbora Křížová & Lenka Drábková & Jaroslava Ovesná, 2017. "Detection of PCR inhibition in food and feed with a synthetic plasmid," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 35(2), pages 160-164.
    • Handle: RePEc:caa:jnlcjf:v:35:y:2017:i:2:id:374-2016-cjfs
    DOI: 10.17221/374/2016-CJFS
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    References listed on IDEAS

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    1. Dimitra HOUHOULA & Polytimi DIMITRIOU & Gena MENGJEZI & Vasiliki KYRANA & Vladimiros LOUGOVOIS, 2015. "Quantification of parvalbumin in commercially important Mediterranean seafood species using real time PCR," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 33(2), pages 143-147.
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