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Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR

Author

Listed:
  • Lucie VONDRÁKOVÁ

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic)

  • Sabina PURKRTOVÁ

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic)

  • Jarmila PAZLAROVÁ

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic)

  • Kateřina DEMNEROVÁ

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic)

Abstract

The differentiation of thermotolerant Campylobacter spp. on the species level (C. jejuni, C. coli, C. lari, and C. upsalien-sis) was provided. Identification is based on different banding patterns obtained for individual species during simple multiplex PCR where regions within the 23S rRNA gene are amplified using newly designed specific forward primers.

Suggested Citation

  • Lucie VONDRÁKOVÁ & Sabina PURKRTOVÁ & Jarmila PAZLAROVÁ & Kateřina DEMNEROVÁ, 2015. "Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 33(1), pages 27-31.
    • Handle: RePEc:caa:jnlcjf:v:33:y:2015:i:1:id:267-2014-cjfs
    DOI: 10.17221/267/2014-CJFS
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