Methanol Fraction Of Ethanol Extract Of Dialium Guineense Stem Bark May Alter The Activity Of Glucose 6-Phosphatase/Aminotransferases And Levels Of Lipids In Tissues Of Diabetic Wistar Rats

The aim of the present study was to investigate the potential of methanol fraction of ethanol extract of Dialium guineense (MEDG) stem bark to alter the activity of glucose 6-phosphatase (G6Pase)/aminotransferases and levels of lipids in tissues of streptozotocin (STZ)-induced diabetic Wistar rats. Adult male Wistar rats (n = 25, mean weight = 215 ± 15 g) were randomly assigned to five groups (5 rats/group): normal control, diabetic, metformin, extract (200 mg/kg body weight, bwt) and extract (300 mg/kg bwt) groups. A single intraperitoneal injection of 50 mg/kg bwt STZ was used to induce diabetes mellitus (DM) in the experimental rats. The diabetic rats were subsequently treated for 21 days with 50 mg/kg bwt metformin (standard antidiabetic agent) or MEDG stem bark. Activities of G6Pase, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and levels of total cholesterol (TC), triacylglycerol (TG), very low-density lipoprotein cholesterol (VLDL-C), and nitric oxide (NO) were measured in plasma or hepatic/cardiac/renal tissues. The results showed that the G6Pase activity that was significantly increased in the plasma and hepatic/renal tissues of rats were markedly reduced after treatment with MEDG stem bark (p 0.05). However, treatment of the diabetic Wistar rats with MEDG stem bark significantly reduced the hepatic activities of ALT and ALP (p