Isolation and Characterization of Arylamidase-Producing Pseudomonas Alcaligenes from the Water of Al-Asfar Lake, Al Ahsa Oasis, Saudi Arabia

Al-Asfar Lake is an important shallow lake that suffered greatly from anthropogenic eutrophication; therefore, it received considerable interest for economic and environmental purposes. Therefore, the current study aimed to investigate the presence of P. alcaligenes based on culture-dependent methods and detect this enzyme activity by the VITEK biochemical tests. The water sample was collected from the lake, serially diluted through sterile saline, and then inoculated and spread onto CHROMagar media and Mueller Hinton agar media. Then purified by the single selection of all different types of colonies by streaking to obtain well-isolated colonies The P. alcaligenes and the enzyme has been successfully identified with a probability of 95% by VITEK 2® COMPACT automated system after determining the Gram stain characteristic for the isolate under a microscope This study proved the existence of P. alcaligenes species that have never been reported previously in Al-Asfar Lake. Therefore, P.alcaligenes species can be an ideal candidate to take advantage of in pollutant biodegradation and to be invested in bioremediation applications to enhance environmental sustainability, in the future.How to Cite: Abeer T. Al Mousa, Ashraf Y. Khalifa, Munirah F. Aldayel, 2022. "Isolation and Characterization of Arylamidase-Producing Pseudomonas Alcaligenes from the Water of Al-Asfar Lake, Al Ahsa Oasis, Saudi Arabia." Journal of Agriculture and Crops, vol. 9, pp. 122-129.