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Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

Author

Listed:
  • Nor Yasmin M. F.

    (Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Selangor Darul Ehsan, Malaysia)

  • Norrizah J. S.

    (Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Selangor Darul Ehsan, Malaysia)

  • Azani S.

    (Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Selangor Darul Ehsan, Malaysia)

Abstract

A study was conducted to determine the effects of different plant growth hormones towards in vitro regeneration of a new Malaysian rice variety (Oryza sativa L.Var. MRIA 1). Stem explants of 4-week- old aseptic seedlings were selected and a total of 16 different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth hormones for in vitro regeneration of Oryza sativa L. Var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/l NAA and 0.5 mg/l Kn with an average of 8.90 ± 1.79 number of shoots, 114.00 ± 18.90 number of roots, 10.70 ± 2.62 cm length of shoots and 8.70 ± 1.64 number of leaves per explant. It was proved that the in vitro regeneration of Oryza sativa L. Var. MRIA 1 from stem explants was achieved without entering the intermediate callus phase within 4 weeks of culture. Thus, laborious sub-culturing processes were not required to regenerate the multiple shoots. These findings provided an optimum plant growth hormone concentration for the tissue culture system of Oryza sativa L. Var. MRIA 1. Acclimatization of regenerated plantlets of Oryza sativa L. Var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol for the variety.

Suggested Citation

  • Nor Yasmin M. F. & Norrizah J. S. & Azani S., 2016. "Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants," Journal of Advances in Technology and Engineering Research, A/Professor Akbar A. Khatibi, vol. 2(4), pages 125-133.
    • Handle: RePEc:apb:jaterr:2016:p:125-133
    DOI: 10.20474/jater-2.4.4
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