Study on Virus-free Breeding Technology of Potato Microtuber

It is the simplest, fastest and effective way to improve the yield level of potato by selecting high quality virus-free seed potato for field production and maintaining the original characters of virus-free minituber. Microtuber is featured by small volume, no pathogen, convenient storage and transportation, which can be induced in summer with high temperature and humidity, and overcome a series of problems such as poor survival rate of transplanted test-tube plantlets, and easy pollution of cutting tips. Therefore, it can replace conventional test-tube plantlets directly for reproduction. Meantime, if minitubers are sown ahead of time using cold frame, the growth stage of potato minituber will avoid the occurrence period of aphids. The use of gauze cover for insect prevention has good effect on maintaining the excellent characters of microtuber. The yield and quality of virus-free potato are greatly improved by means of virus-free culture of shoot tip seedlings, subculture propagation of virus-free seedlings, induction of microtubers by dark culture, cultivation of breeder’s seeds in greenhouse and screenhouse to preserve the characters of potato.