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Fluorescence-based PCR Detection of Goat Milk Powder Mixed with Cow milk

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  • WANG, Yunxia
  • JING, Lijuan
  • LI, Zhijun
  • LI, Cuizhi
  • LU, Zhiyong
  • LIU, Lijun

Abstract

[Objectives] To amplify and identify the specific gene fragments of cattle and sheep by real-time fluorescence-based PCR, and quickly and accurately identify the adulteration problem of goat milk powder according to the differences of mitochondrial genes among different species. [Methods] The specificity, limit of detection and repeatability test were carried out by isolating somatic cells, extracting template DNA and determining the reaction conditions of real-time fluorescence-based PCR. [Results] The real-time fluorescence-based PCR method could be used to identify the bovine and ovine-derived materials of milk powder with high specificity, high sensitivity and good repeatability, and the limit of detection of milk components in goat milk powder could reach 0.01%. [Conclusions] The real-time fluorescence-based PCR method can be used to quickly and accurately detect the milk components in goat milk powder, and provide some technical support for the adulteration detection of dairy industry.

Suggested Citation

  • WANG, Yunxia & JING, Lijuan & LI, Zhijun & LI, Cuizhi & LU, Zhiyong & LIU, Lijun, 2021. "Fluorescence-based PCR Detection of Goat Milk Powder Mixed with Cow milk," Asian Agricultural Research, USA-China Science and Culture Media Corporation, vol. 13(08), August.
    • Handle: RePEc:ags:asagre:317691
    DOI: 10.22004/ag.econ.317691
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    Keywords

    Agribusiness;

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