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Preservation And Maintenance Of Virus-Free Clonal Citrus Germplasm At The National Clonal Germplasm Repository For Citrus, Riverside, California

Author

Listed:
  • Williams, T.E.

Abstract

The United States Department of Agriculture's National Clonal Germplasm Repository for Citrus is currently establishing a virus-free clonal germplasm collection of 830 accessions of citrus and related Aurantioideae genera at its Riverside, California, facility with the objective of reducing genetic vulnerability of the crop and providing a source of virus-free clonal germplasm to researchers worldwide. Emphasis of the collection centers on the acquisition and ex-situ preservation, maintenance, evaluation and distribution of genera, species and cultivars representing the widest possible genetic diversity with the citrus subfamily. The Repository plan includes a comprehensive program of genetic characterization, pathogen detection and elimination, pest management, propagation in a controlled and protected environment, and documentation. All accessions are evaluated for trueness-to-type using leaf isozymes and characterized for 58 descriptors. Research is expanding into RFLPs for the development of an usable genomic map. The pathogen detection program evaluates accession for 9 virus and viroid and 2 bacterial diseases. Infected accessions receive shoot-tip micrografting and thermotherapy to eliminate pathogens. All accessions are maintained as potted trees in aphid-proof screen- or greenhouses. Citrus seed, pollen and buds are being investigated for extended preservation under lowtemperature and cryostorage. Research investigations into in vitro culturing of explants have begun. A complete computerbased record of each accession is maintained on site and in the USDA-ARS Germplasm Resources Information Network (GRIN).

Suggested Citation

  • Williams, T.E., 1990. "Preservation And Maintenance Of Virus-Free Clonal Citrus Germplasm At The National Clonal Germplasm Repository For Citrus, Riverside, California," 26th Annual Meeting, July 29 to August 4, 1990, Mayaguez, Puerto Rico 259337, Caribbean Food Crops Society.
    • Handle: RePEc:ags:cfcs90:259337
    DOI: 10.22004/ag.econ.259337
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